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the transfection efficiency of the cho-s cells was higher than that of the other cell lines tested. protein expression was induced with 0.5 μg/ml tetracycline (sigma) for 48 h and the amount of the cmg2-fc mutant was measured by enzyme-linked immunosorbent assay (figure. 3). in order to determine whether the cmg2-fc mutant was the main component of the supernatant, the cmg2-fc mutant was purified by protein a column from the supernatant of cho-s cells expressing cmg2-fc mutant. the purity of the cmg2-fc mutant was approximately 98% as measured by sds-page and western blotting (figure. the purified cmg2-fc mutant showed the same molecular weight as the expressed protein, and the biological activity of the purified cmg2-fc mutant was confirmed by elisa assay figure. 1 and the cmg2-fc mutant was expressed at a high level in cho-s cells. the cmg2-fc mutant is comprised of amino acids 1225 of human cmg2, followed by the hinge and fc regions of human igg1 (the c-terminal 232 amino acids of igg1). the molecular weight of this fusion was approximately 47 kda under reducing conditions (consistent with cmg2-fc) as determined by mass spectroscopy (data not shown). the expression of protein was identified by western blot analysis using mouse anti-cmg2 polyclonal antibody (anti-antxr2 antibody ab70499, abcam, uk).

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the cmg2-fc mutant can be cloned into the retroviral vector csgw to produce a high-titre retroviral vector that can transduce mammalian cells. the retroviral vector was then used to transfect cho-s cells to produce recombinant retrovirus. the cells were then expanded in serum-free dmem/f12 medium. the recombinant retrovirus can be packaged into the retroviral particles and infect the target cells. the cells were then selected by puromycin. after puromycin screening, we got the stably transfected cell line. the cmg2-fc was purified from the culture medium by protein a affinity chromatography. the recombinant protein was identified by sds-page and western blotting. the recombinant protein is composed of the vwa domain of cmg2 and the fc region of human igg1 (the c-terminal 232 amino acids of igg1) (figure. 3). the molecular weight of this fusion was approximately 47 kda under reducing conditions (consistent with cmg2-fc) as determined by mass spectroscopy (data not shown). the expression of protein was identified by western blot analysis using mouse anti-cmg2 polyclonal antibody (anti-antxr2 antibody ab70499, abcam, uk).
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