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The incidence of AmpC-type beta-lactamases in Enterobacteriaceae in Japan was evaluated during 1997-1999, by screening for the AmpC phenotype. AmpC strains were recovered from a total of 7,385 isolates (0.91%), of which AmpC-3 strains were most common (1.5%), followed by AmpC-4 (0.83%), AmpC-1 (0.51%), and AmpC-6 (0.15%). The major sources of AmpC-3 strains were Escherichia coli (88.0%) and Proteus mirabilis (14.2%). Of the 53 AmpC-producing E. coli isolates, 38 (71.7%) were CTX-M-type enzymes, whereas only 2 (3.8%) were TEM-type enzymes. Most of the AmpC-3 and -4 strains had a chromosomal location of ampC, while the ampC gene was extrachromosomal in AmpC-1 and AmpC-6 strains. All the AmpC-6 strains, containing the ampC gene and the ampD gene, belonged to the K12 serotype. Many AmpC-positive strains with plasmid(s) (as well as non-plasmid-encoded AmpC-positive strains) were isolated. All AmpC-positive strains were not selected by plasmid-encoded AmpC inhibitor. These results showed that AmpC-producing strains were widely distributed in Japan and that the major source was the faeces of urinary tract patients, suggesting that AmpC-producing Enterobacteriaceae were common in Japan.Fluorine is known as a very stable, inert and nonvolatile element in the periodic table of elements, and yet is very reactive with itself and most other elements. Elemental fluorine is a gas at room temperature, and a liquid below 100.degree. C. In the solid state, fluorine exists as a hexafluoride.
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