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Three-day-old mice were sensitized with 100 micrograms of anti-TNP IgG, and their serum was collected and their erythrocytes were washed and injected into mice. The mice were sacrificed and the levels of the nucleosides in the erythrocytes, serum and organs were measured and the titer of their anti-TNP antibody was determined by hemagglutination inhibition and ELISA method. At 4 days after sensitization, the levels of the nucleosides in the erythrocytes were significantly decreased and the titer of anti-TNP antibody increased. The levels of the nucleosides in the organs were relatively decreased, and the correlation between the erythrocyte nucleosides and the titer of the antibody was weak. These results suggest that a decreased level of the nucleosides in erythrocytes induced the production of the antibody and that also contributed to the development of the anemia.Chromatographic characterization and photo-induced lysis performance of aqueous two-phase system involving dextran and polystyrene sulfonate.
The objective of this study is to characterize an aqueous two-phase system (ATPS) consisting of dextran (DX) and polystyrene sulfonate (PSS) using low-pressure chromatography. Sedimentation partition coefficients of DX, PSS and a polymer in an ATPS were determined using the general equation for partition coefficients and the operational stability of the ATPS was monitored by the release rate of DX and PSS from the top phases. The performance of the ATPS was evaluated using different dextran and polystyrene sulfonate concentrations and the polymerization degree of DX. The performance of the ATPS was characterized using the polymer concentration profiles in the top and bottom phases. The chromatographic performance of the ATPS revealed that the top phase (top PSS in this case) exhibited a better chromatographic resolution compared with the bottom phase (bottom PSS), which implies that the first-order rate constant for the release of DX (kDX) from the
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